Part:BBa_K1333108

DNAQ926 CDS

DNAQ926 coding sequence,lead to polymeraseIII proofreading defect in E.coli.

Usage and Biology

DNA polymerase III is the main enzyme during DNA replication. DNAQ encodes the epsilon subunit of DNA polymerase III which is responsible for the proofreading function. DNAQ has the 3’-5’ exonuclease activity. During the process of DNA replication ,as soon as base mispairing occur, DNAQ will cut off the newly synthesized base, which enable the right base to substitute its position. This guarantee the accuracy of DNA replication. DNAQ926 is a mutant of DNAQ, which doesn’t have the proofreading function but still can bind to the polymerase III complex. If DNAQ926 is expressed in the E.coli, it can compete the internal product of DNAQ, which can lead to the mutation during DNA replication.

defective proofreading

For more information please visit our wiki

http://2014.igem.org/Team:SYSU-China/content.html#Project/Result/Mutagenesis

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 174
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 46
  Illegal AgeI site found at 535
  
• 1000
  COMPATIBLE WITH RFC[1000]